Can using up other PCIe 1x slots affect GPU performance on the first

Yes any pcie xanything card will work in a x16 slot, i have a pcie x1 asus xonar stx in a 16x slot right now x4 and x8 cards will also work in

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pcie slot qrelease EXPANSION CHASSIS FOR PCIE CARDS: Increase the PCI-e slot count of a desktop computer or server; Add four external PCIe x1 slots;

pcie slot configuration gen1 gen2 gen3 pci e 1x slot riser,Despite the result, both teams displayed great sportsmanship and camaraderie, showcasing the true spirit of the sport